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Volume 10 Issue 3
June 1995
    Citation: WU Yun-Feng, WEI Ning-Sheng. Purification and Specific Characterization of Potyvirus Helper Component .VIROLOGICA SINICA, 1995, 10(3) : 269.
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    Purification and Specific Characterization of Potyvirus Helper Component

    • 1.

      The state key library of Biology for Plant Desease and Insect Pests,Beijing 100094

    • Available online: 05 September 1995
    • Two helper components(HC)were purified with PEG precipitation and DEAE-cellulosechromatography from plants infected by tobacco etch virus(TEV)and watermelon mosaicvirus 2(WMMV-2).Bioassay results revealed that the Myzus persicae and Aphis gossypii cantransmit TEV after acquiring first TEV plus HC-TEV of HC-TEV,then TEV by parafilmmembrane method.HC encoded by WMMV-2 can assist aphids to transmit WMMV-2,butnot TEV,PAV-BYDV and tobacco mosaic virus(TMV).
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      Purification and Specific Characterization of Potyvirus Helper Component

      • 1. The state key library of Biology for Plant Desease and Insect Pests,Beijing 100094

      Abstract: Two helper components(HC)were purified with PEG precipitation and DEAE-cellulosechromatography from plants infected by tobacco etch virus(TEV)and watermelon mosaicvirus 2(WMMV-2).Bioassay results revealed that the Myzus persicae and Aphis gossypii cantransmit TEV after acquiring first TEV plus HC-TEV of HC-TEV,then TEV by parafilmmembrane method.HC encoded by WMMV-2 can assist aphids to transmit WMMV-2,butnot TEV,PAV-BYDV and tobacco mosaic virus(TMV).

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