Experimental Studises Oil Viral Hepatitis Receptor——targeted Drug L——HSA-AraAMP

Lactosaminated Human Serum Albumin(L-HSA)was,used as a carrier and coupled to Ara-binosied adenine monophospate(AraAMP).The distribution of 125I-labelled L-HSA-AraAMPwere higher in liver than in other organs.Radioactivities of the drug could still be measured atthe 7th dav after injection.The efficacy of targeted drug was determined by analyzing dynamic changes of HBsAg andHBeAg in 2.2.15 cell culture medium by RIA and changes in the replicative intracellular HBVDN A content by Southern blot.The results showed that the targeted drug had significantly in-hibitory ratio of HBsAg and HBeAg which was similar to AraAMP(inhibitory ratfo of HBsAg50%,63%;HBeAg 62.6%,67.2%,respectively).Southern blot test showed the replicativeintermedlates rcDNA and ssDNA were inhibited by AraAMP.The targeted drug had parallel an-ti-H BV DN A reDlicative effectiveness com pared with AraAMP.The values of Si of drugs toHBsAg and HBeAg were detected.