Citation: ZHANG Hai-Bao, ZHU Xi-Ru, ZHANG Yun-Kai. Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum .VIROLOGICA SINICA, 1995, 10(4) : 367.

Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum

  • Available online: 05 December 1995
  • The virus isolate S_1 was isolated from chrysanthemum plants with the symtoms of viral dis-ease,The host range of isolate S_1 was wide.It could infect 11 species plants aniong tested 6 fami-lies by sap-inoculation, The isulate S_1 was also transmitted in non-persistant manner by Myzuspersicae,The rate of transmission was 86.7%(13/15).The vlrus particle of isolate S_1 wassphere stiape, ItS diameter was about 28-30nm,The serological test showed that isolate S_1 re-acted positively with the antiserum against CMV but negatively with ToAV antiserum.On thebasis of these characteristics,isolate S1 was identified as a member of cucumber mosaic virus(CMV),The rabbit antiserum against CMV(isolate S1)was prepared with purified isolate S1.The highest titer of the antiserum was 1:2400 tested by ELISA.All 158 virus disease samples of field chrysanthemum plants were detected with the prepared antiserum by indirect-ELISA,inwhich 19.0% amples showed positive reaction.

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    Prelim inary Identification Cucumber Mosaic Virus and Serological Detection of Infecting Chrysanthemum

    • 1. China South Institute of Botang, Academia Sinica, Guangzhou 510650

    Abstract: The virus isolate S_1 was isolated from chrysanthemum plants with the symtoms of viral dis-ease,The host range of isolate S_1 was wide.It could infect 11 species plants aniong tested 6 fami-lies by sap-inoculation, The isulate S_1 was also transmitted in non-persistant manner by Myzuspersicae,The rate of transmission was 86.7%(13/15).The vlrus particle of isolate S_1 wassphere stiape, ItS diameter was about 28-30nm,The serological test showed that isolate S_1 re-acted positively with the antiserum against CMV but negatively with ToAV antiserum.On thebasis of these characteristics,isolate S1 was identified as a member of cucumber mosaic virus(CMV),The rabbit antiserum against CMV(isolate S1)was prepared with purified isolate S1.The highest titer of the antiserum was 1:2400 tested by ELISA.All 158 virus disease samples of field chrysanthemum plants were detected with the prepared antiserum by indirect-ELISA,inwhich 19.0% amples showed positive reaction.

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