Transformation of spleen cells prepared from dengue virus-2 immunized hu-TLC-SCID mice

The spleen cells isolated from SCID mice transplanted with human tonsillar lymphocytes(Hu- TLC-SCID)immunized with dengue virus 2 type(DV_2)in vitro and in vivo were trans-formed by Epstein-Barr virus(EBV).Specific human anti-DV_2 antibodies were detectable in thesupernatant of the transfomed spleen cells.The presence of human B lymphocyte markers(and SmIgG) as well as EBV antigens (LMP1 and BENA)and LMP1 gene fragment in the trans-formed spleen cells determined by immunoperoxidase staining immunofluorescence technique andPCR technique,indicated that the Hu-TLC-SCID mouse spleen cell has been successfully trans-foimed by EBV.The EBV-transformed Hu-TLC-SCID mouse spleen cell could be utilized forcontinous production of specific human anti-DV_2 antibodies in vitro.