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Volume 11 Issue 2
April 1996
    Citation: WANG Su-Yan, CHENG Gang, LIU Wei-Ping, XIE Yin, TIAN Bo. Construction of rice expression vector including antisense ribozyme and partial sequence of repli- case in rice dwarf virus .VIROLOGICA SINICA, 1996, 11(2) : 164.
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    Construction of rice expression vector including antisense ribozyme and partial sequence of repli- case in rice dwarf virus

    • 1.

      Institute of Microbiology ,Chinese Academy of Sciences

    • Available online: 05 June 1996
    • he whole sequence of RDV S1 fragment and partial sequence(S5Ⅲ)of RDV S5 fragmentwere synthesized and amplified by the method of reversed transcription-polymerase chain reaction(RT-PCR).Each amplified product was then cloned into pGEM7Zf(+)or pUC19 at the Smalsite,and sequenced.Based on these results,a hammer-head ribozyme sequence was introduced intoantisense chain of S5Ⅲfragment to construct antisense ribozyme gene S5ⅢR,Rice transformationvector pROK-S1-1' and pROK-S5ⅢRwere constructed by inserting S5ⅢRgene and 5'-end se-quence of S1 fragment(S1-1')into plant expression vector pROK Ⅱ.
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      Construction of rice expression vector including antisense ribozyme and partial sequence of repli- case in rice dwarf virus

      • 1. Institute of Microbiology ,Chinese Academy of Sciences

      Abstract: he whole sequence of RDV S1 fragment and partial sequence(S5Ⅲ)of RDV S5 fragmentwere synthesized and amplified by the method of reversed transcription-polymerase chain reaction(RT-PCR).Each amplified product was then cloned into pGEM7Zf(+)or pUC19 at the Smalsite,and sequenced.Based on these results,a hammer-head ribozyme sequence was introduced intoantisense chain of S5Ⅲfragment to construct antisense ribozyme gene S5ⅢR,Rice transformationvector pROK-S1-1' and pROK-S5ⅢRwere constructed by inserting S5ⅢRgene and 5'-end se-quence of S1 fragment(S1-1')into plant expression vector pROK Ⅱ.

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