The Pr~luetion ofTNF-~ I~dueed by HI-IV ——6 in Vitro

here are high levels of TNF-α in the supernatants of lymphocyte culture infected with HHV-6 GS strain and the Nanjing loeal strains.TNF-α was detected by two methods:biological ac-tivity assay using L929 cells and ELISA.HHV-6 can induce TNF-α with in 24 h,maximal re-lease of TNF-α occurred during 48 h to 72 h post infection and then gradually decreased.The levelof TNF-α in infected culture suprenatants is far higher than that of uninfecter cells(P0.1).Three lo-cal strains have same result.Specific monoclonal antibody against TNF-α can completely neutralizethe activity of TNF in the supernatants of these cells infected with HHV-6.The levels of TNF-αinduced by HHV-6 is much higher than that induced by LPS as a positive control.