Isolation and Identification of an Avian Reovirus Variant Strain
Abstract: virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only on chicken embryo liver cell(CELi)but not on CEF and Vero cell,andwas inactive at 50℃ for 1 hour,even in Mg ̄(2+)solution.Additionally,the migration rate of foursmall segments of it's genome was slightly different from ARV FDO and S_(1133) strain.The experi-ment suggested that the virus is a variant strain of ARV.