Citation: DIAO Zhen-Fen, XU Wei-Yan, DU Nian-Xin, XUE Heng-Beng. Isolation and Identification of an Avian Reovirus Variant Strain .VIROLOGICA SINICA, 1996, 11(4) : 352.

Isolation and Identification of an Avian Reovirus Variant Strain

  • Available online: 05 December 1996
  • virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only on chicken embryo liver cell(CELi)but not on CEF and Vero cell,andwas inactive at 50℃ for 1 hour,even in Mg ̄(2+)solution.Additionally,the migration rate of foursmall segments of it's genome was slightly different from ARV FDO and S_(1133) strain.The experi-ment suggested that the virus is a variant strain of ARV.

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    Isolation and Identification of an Avian Reovirus Variant Strain

    • 1. College of Animal Medica1.Nanjing Agriculture University,Nanjing 210095

    Abstract: virus strain was isolated from the liver of a sick chicken and identified to be an avian re-ovirus(RV)by observation of electron-microscopy,analysis of physical-chemieal characteris-tics,SDS-PAGE of nucleic acid and neutralization test.The virus strain produced evident cyto-pathic effect(CPE)only on chicken embryo liver cell(CELi)but not on CEF and Vero cell,andwas inactive at 50℃ for 1 hour,even in Mg ̄(2+)solution.Additionally,the migration rate of foursmall segments of it's genome was slightly different from ARV FDO and S_(1133) strain.The experi-ment suggested that the virus is a variant strain of ARV.

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