Citation: CHEN Yu-Guang. Molecular cloning of highly reiterated sequence AGMr (Hind I ) - 1 in Vero cell line of African green, monkey and its preliminary application .VIROLOGICA SINICA, 1997, 12(2) : 124.

Molecular cloning of highly reiterated sequence AGMr (Hind I ) - 1 in Vero cell line of African green, monkey and its preliminary application

  • Available online: 05 June 1997
  • Highly reiterated Sequence AGMr (Hind Ⅲ)-l was isolated from genome of Vero cell line derived from African green monkey and cloned into plasmid pUC18. Sequence analysis confirmed that the insert Sequence is right but 6 out of 172 bp had mutated in the 165th generation of Vero cell. Southern Blot and Dot Blot analysis of rabies vaccine prepared by Vero cell showed that residual cell DNA(RC DNA) displayed heterogeneous size from 400 ~ 5000 bp, and that the amount of RCDNA can be determined specifically and sensitively (minimun 4 pg). The fact that AGMr (Hind Ⅲ )-l Sequence represents about 20% of the genome DNA and repeats 6.8 × 106times and is rare in Primates except in members of the same genus also suggested that the recombinant plasmid could be applied as probe for detecting RC DNA existed in vaccine prepared by Vero cell and maybe used for identifying test of Vero cell.

  • 加载中
  • 加载中

Article Metrics

Article views(4081) PDF downloads(747) Cited by()

Related
Proportional views

    Molecular cloning of highly reiterated sequence AGMr (Hind I ) - 1 in Vero cell line of African green, monkey and its preliminary application

    • 1. Shanghai lnstitute of Biological Products.Shanghai 200052

    Abstract: Highly reiterated Sequence AGMr (Hind Ⅲ)-l was isolated from genome of Vero cell line derived from African green monkey and cloned into plasmid pUC18. Sequence analysis confirmed that the insert Sequence is right but 6 out of 172 bp had mutated in the 165th generation of Vero cell. Southern Blot and Dot Blot analysis of rabies vaccine prepared by Vero cell showed that residual cell DNA(RC DNA) displayed heterogeneous size from 400 ~ 5000 bp, and that the amount of RCDNA can be determined specifically and sensitively (minimun 4 pg). The fact that AGMr (Hind Ⅲ )-l Sequence represents about 20% of the genome DNA and repeats 6.8 × 106times and is rare in Primates except in members of the same genus also suggested that the recombinant plasmid could be applied as probe for detecting RC DNA existed in vaccine prepared by Vero cell and maybe used for identifying test of Vero cell.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return