Establishment of method for detection of respiratory syncytical virus RNA using reverse- transcription polymerase chain reaction
Abstract: A method of reverse - transcription polymerase chain reaction(RT - PCR) was established for detection of respiratory syncytical virus (RSV) RNA genome, The primers were designed based on the nucleotide sequence of RSV N gene. The results showed that the primers can't amplify the genomes of influenza virus and parainfluenza virus, but it can detect RSV genome in naSopharyngeal aspirate samples in infants and young children. This method is better than virus isolation. Its specificity is similar to immunofluorescence assay. The results indicated that the RTPCR for RSV RNA is specific, rapid and sensitive. It is suitable for clinical diagnosis of RSV infection.