Citation: LI Gang, YAO Ji-Lu, CHEN Qing, PENG Wen-Wei, TANG Wen-Hui. Sequence analysis of GBV- C/HGV 5' non- coding region cDNA .VIROLOGICA SINICA, 1997, 12(3) : 224.

Sequence analysis of GBV- C/HGV 5' non- coding region cDNA

  • Available online: 05 September 1997
  • The cDNA sequence of 5' non - coding region (5'NCR) of GBV - C/HGV strain from China was determined. According to the previously reported sequences of HGV, two pairs of primers specific for HGV from relatively conserved regha in the 5' NCR was designed- GBV - C/HGV RNA derived from the plasma of a intravenous drug user in Kunming district (Yunnan Province,China) was extracted using the heat denaturization method and was subsequently converted to cD-NA by reverse transcription before nested RCR. The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method. A Comarison of the nucleotlde sequence of GBV - C/HGV from China with that of several previously reported isolates from abroad showed the homology to be 86. 36 % ~ 90. 91 % and a divergent region. The results demonstrated that the amplified fragment was derived from the genome of GBV - C/HGV. The sequence of the GBV - C/HGV 5' NCR determined here was useful for the selection of primers and for the

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    Sequence analysis of GBV- C/HGV 5' non- coding region cDNA

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    Abstract: The cDNA sequence of 5' non - coding region (5'NCR) of GBV - C/HGV strain from China was determined. According to the previously reported sequences of HGV, two pairs of primers specific for HGV from relatively conserved regha in the 5' NCR was designed- GBV - C/HGV RNA derived from the plasma of a intravenous drug user in Kunming district (Yunnan Province,China) was extracted using the heat denaturization method and was subsequently converted to cD-NA by reverse transcription before nested RCR. The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method. A Comarison of the nucleotlde sequence of GBV - C/HGV from China with that of several previously reported isolates from abroad showed the homology to be 86. 36 % ~ 90. 91 % and a divergent region. The results demonstrated that the amplified fragment was derived from the genome of GBV - C/HGV. The sequence of the GBV - C/HGV 5' NCR determined here was useful for the selection of primers and for the

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