Expression of Hantaan Virus Envelope Glyeoproteins Gl and G2 in E .coli

Tlle expression vector plasmid pBV220 was used tO expre~ ,the envelope曹lycuproteins，G1 andG2 ofFtantsall~vil-uJ5inE．co／i．First，the codingframegenesofG1 andG2werem plifiedby PCR，and doned into the PCR product cloning Tvector，then they；were cut f u蜕|唱 抟s韵曲蛹n endonudeases from the T～vector cloning plasmids．Last．the expression pl of G1^ G2 were constixtcted llgatingthe oondingframesofG1 andG2with眈 曲iDn豫呲 蛐．The expressed proteins could not be seen on SDSPAGE ge1．Tbey coutd he reacted with part of mono clonal antibodies against Hantaan virus G1 and G2．but not he t墨ted in We|tem ，bI卧．Mice were immunized with expressed Gl and G2．an tibody ag ainst Han ~ n V~t／S。0uld he ~sted in the sera of the immunized mice．the IFA(Indirect 1：160 and l：320，respectively． Assay)antibody titers were l