Citation: CHEN Zhuang, DU Yong, BO Wen-Sheng, WANG Xue, WANG Hai-Tao. Cloning, Sequencing and Expressing of HCV NS 4 Gene in E. coli .VIROLOGICA SINICA, 1997, 12(4) : 329.

Cloning, Sequencing and Expressing of HCV NS 4 Gene in E. coli

  • Available online: 05 December 1997
  • NS 4 gene of hepatitis C virus was amplified by RT - PCR using RNA template isolated from anti - HCV positive serum of a blood donor from Hebei province in China. The amplified fragmint (abeut 900bp)was cloned into plasmid pET17Xb and the recombinant plasmid was des-ignated pETNS 4. SDS - PAGE analyses showed that a 65 ari fused protien was expressed in E.coli BL21 (DE3 ) harboring pETNS 4 and the expression level of the protien was 24 %. The cDNA fragment of insert of pETNS 4 was sequenced with the dideoxy...

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    Cloning, Sequencing and Expressing of HCV NS 4 Gene in E. coli

    • 1. Institute of Microbioloigy and Epidemiology

    Abstract: NS 4 gene of hepatitis C virus was amplified by RT - PCR using RNA template isolated from anti - HCV positive serum of a blood donor from Hebei province in China. The amplified fragmint (abeut 900bp)was cloned into plasmid pET17Xb and the recombinant plasmid was des-ignated pETNS 4. SDS - PAGE analyses showed that a 65 ari fused protien was expressed in E.coli BL21 (DE3 ) harboring pETNS 4 and the expression level of the protien was 24 %. The cDNA fragment of insert of pETNS 4 was sequenced with the dideoxy...

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