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Volume 12 Issue 4
August 1997
    Citation: LI Hong-Wei, TU Chang-Chun, LV Zong-Ji, JIN Kuo-Shi, YAN Shen. Sequence Analysis and Comparison of gp55 antigen encoding region of Hog Cholera Virus isolates of Changchun and Jilin .VIROLOGICA SINICA, 1997, 12(4) : 354.
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    Sequence Analysis and Comparison of gp55 antigen encoding region of Hog Cholera Virus isolates of Changchun and Jilin

    • 1.

      Changchun University of Agricultural and Animal Sciences

    • 2.

      Changchun

    • Available online: 05 December 1997
    • One pair of oligonucleotide primers were designed to amplify the region of hog cholera virus (HCV)genome, which corresponds to a 588 bp portion encoding the major protective antigen re-gion, a part of gp55 protein. The product was amplified from two virus isolates (strains CC and JL)which had been responsible for two hog cholera outbreaks in Jilin province in the northeast of China. The cDNA products were cloned into pGEM - T vector. Nucleotide sequencing was per-formed by Sanger s method. A part of the obt...
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      Sequence Analysis and Comparison of gp55 antigen encoding region of Hog Cholera Virus isolates of Changchun and Jilin

      • 1. Changchun University of Agricultural and Animal Sciences
      • 2. Changchun

      Abstract: One pair of oligonucleotide primers were designed to amplify the region of hog cholera virus (HCV)genome, which corresponds to a 588 bp portion encoding the major protective antigen re-gion, a part of gp55 protein. The product was amplified from two virus isolates (strains CC and JL)which had been responsible for two hog cholera outbreaks in Jilin province in the northeast of China. The cDNA products were cloned into pGEM - T vector. Nucleotide sequencing was per-formed by Sanger s method. A part of the obt...

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