Comparison and Analysis on HCV NS5 Gene Fragment between GenotypeⅡ isolates from Same Region
Abstract: Isolates from two patients of hepatitis C in the same region were classified as genotype Ⅱ by PCR method. NS5 gene fragments of HCV were cloned, and inserted into pUC19 and M13mp18, respectively.Sequence analysis showed that the homologies of the two isolates (NS5X and NS5V) in nucleotide and amino acid are 89.4% and 90.75%,respectively. Stop codon TGA was found in NS5V at 70～72 nt. The homologies of nucleotide and amino acid between the two isolates and typical genotype Ⅱ(HCV BK)are 91.5%and 91.92%,91.91% and 91.91%. The results indicated that there is relatively high variation in HCV NS5 region between isolates of same genotype from same area, and suggested that the isolate of NS5V may be a defective virus.