Broadly Imm unological Reativities Primed by Epitopes COrrespOnding to the Cleavage Region of SAll Vp4
Abstract: With the flock house virus (FHV) capsid protein as a vector (FHV RNA 2),the epitopes corresponding to the cleavage regions of rotavirus SA11 Vp4 primed broadly cross reactive neutralizing antibodies in guinea pigs. The epitopes of SA11 Vp4:REA(223～242aa),REB(243～262aa),REC(234～251aa),and their combination REA+REB+REC were expressed on the chimeric protein surface in the recombinant pET FHV RE system,and used to immunize guinea pigs to prepare antisera. The antisera were proven to have strong cross recognizing and cross neutralizing activities to homologous SA11 virus and heterologous Wa,S2,DS 1,Ito Sty 3, St. Thomase, Gottfried, UK, and B223 virus strains. The results suggested that the amino acid sequences of rotavirus Vp4 cleavage region have important immunological functions,and when displayed in the proper positions on the FHV RNA 2 protein vector, these epitopes could be prospective as a new type of epitope based subunit vaccines.