Cloning and Sequencing of the Helicoverpa armigera Nuclear Polyhedrosis Virus DNA Polym erase Gene

The location of DNA polymerase gene in the HaSNPV was determined within a 6.6kb HindⅢ J fragment,using a probe prepared from the previously identified DNA polymerase from the Helicoverpa zea single nuclear polyhedrosis virus(HzSNPV).The entire coding region of HaSNPV DNA pol was in a HindⅢ SalI fragment of 3.4kb in size.The restriction enzyme map of HaSNPV DNA pol was similar to that of HzSNPV. A 806bp fragment in the subclone was sequenced and the partial ORF containing 206aa was predicated. Compared with other six members of Baculoviridae ,the DNA polymerase of HaSNPV was highly homologous with that of HzSNPV,and it also showed certain similarity to those of LdMNPV,AcMNPV,BmNPV,CfMNPV and OpMNPV.This study can supply necessity for further attachment of the replication mechanism of HaSNPV.