Citation: XU Dong-Gang, NIU Jian-Zhang, MENG Zong-Da, DAI Hao-Ying, CHEN Chu-Fen, CUI Ze, SHU Hui-Bin, MENG Wen-Hua. Cloning of human interleukin 18 gene and its expression in E. coli .VIROLOGICA SINICA, 1998, 13(2) : 138.

Cloning of human interleukin 18 gene and its expression in E. coli

  • Available online: 05 June 1998
  • Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The recombinant protein amounts to 27.2% of total bacterial protein.

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    Cloning of human interleukin 18 gene and its expression in E. coli

    • 1. Institute of Basic Medical Sciences,Academy of Military Medical Scieces.Beijing 100850 Hebei Provincial Hygiene and Anti-epidemic Station,Baoding 071D00

    Abstract: Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The recombinant protein amounts to 27.2% of total bacterial protein.

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