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Volume 13 Issue 2
April 1998
    Citation: 彭辉银 李星 张双民 Ｂａｓｉｌ Ｍ．Ａｒｉｆ 陈新文 胡志红, . Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus .VIROLOGICA SINICA, 1998, 13(2) : 139.
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    Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus

    • 1.

      Wuhan Institute of Virology，Chinese Academy Sciences．Wuhan 430071Forest Pest Management Institute，Sault Ste Marie．Canada，P6A5M7

    • Available online: 05 June 1998
    • The chitinase gene of Heliothis armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV) was localized on BamHI E, BglII E,EcoRI G,HindIII F,XbaI H,BamHI+HindIII M,and BamHI+XbaI H fragments, using the α P 32 dATP labelled recombinant plasmid harboring Choristoneura fumiferana multiple nucleocapsid nucleopolyhedrovirus (CfMNPV)DNA HindIII M which contains chitinase gene as the probe. The XbaI H fragment of HaSNPV was cloned into pTZ 19R vector for further sequencing the chitinase gene.
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      Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus

      • 1. Wuhan Institute of Virology，Chinese Academy Sciences．Wuhan 430071Forest Pest Management Institute，Sault Ste Marie．Canada，P6A5M7

      Abstract: The chitinase gene of Heliothis armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV) was localized on BamHI E, BglII E,EcoRI G,HindIII F,XbaI H,BamHI+HindIII M,and BamHI+XbaI H fragments, using the α P 32 dATP labelled recombinant plasmid harboring Choristoneura fumiferana multiple nucleocapsid nucleopolyhedrovirus (CfMNPV)DNA HindIII M which contains chitinase gene as the probe. The XbaI H fragment of HaSNPV was cloned into pTZ 19R vector for further sequencing the chitinase gene.

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