Localization and Cloning of the Chitinase Gene of Heliothis armigera Single Nuclocapsid Nucleopolyhedrovirus
Abstract: The chitinase gene of Heliothis armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV) was localized on BamHI E, BglII E,EcoRI G,HindIII F,XbaI H,BamHI+HindIII M,and BamHI+XbaI H fragments, using the α P 32 dATP labelled recombinant plasmid harboring Choristoneura fumiferana multiple nucleocapsid nucleopolyhedrovirus (CfMNPV)DNA HindIII M which contains chitinase gene as the probe. The XbaI H fragment of HaSNPV was cloned into pTZ 19R vector for further sequencing the chitinase gene.