Nucleotide Sequence of the Helicoverpa armigera Nuclear Polyhedrosis Virus HindIll-K Fragment and Its Analysis

Nucleotide sequence of the Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus (HaSNPV) HindIII K fragment has been sequenced. The fragment comprises 3255 bp, containing 15 ORFs that are at least 40 amino acids in length, including 489 bp of the 3′end of polyhedrin(ph) coding region and complete coding region (801bp) of the protein kinase (HavPk). An open reading frame, ORF 1236, encoding a 412 amino acid polypeptide was indentified between immediately downstream ph gene and upstream HavPK gene, but the transcriptional direction was opposite to them. Comparison of the nucleotide sequences and deduced amino acid sequences demonstrated that the ORF 1236 is closely similar to the ORF8 of H.zea single nuclear polyhedrosis virus (HzSNPV),with 95.9% amino acid identity, but is distantly related to the ORF 1629 of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV),with only 24.8% amino acid identity.