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Volume 13 Issue 2
April 1998
    Citation: LIU Ming-Jun, WANG Zheng-Dang, WANG Sheng-Qi. Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin .VIROLOGICA SINICA, 1998, 13(2) : 159.
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    Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin

    • 1.

      Animal Biotechnology Laboratory，Xinjiang Academy of Animal Science，Urumqi 830000

    • Available online: 05 June 1998
    • According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not hybridize to Group A and Group C rotavirus.The results showed that the probe has high specificity and sensitivity.It can be used to detect and identify GBRV,and also to research the genetic relativity of GBRV gene mutation and rearrangement.
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      Detection of the Group B Rotavirus with the Probe 0f 0lig0nucle0tides Labelled with Biotin

      • 1. Animal Biotechnology Laboratory，Xinjiang Academy of Animal Science，Urumqi 830000

      Abstract: According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not hybridize to Group A and Group C rotavirus.The results showed that the probe has high specificity and sensitivity.It can be used to detect and identify GBRV,and also to research the genetic relativity of GBRV gene mutation and rearrangement.

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