Analysis of Fatty Acids in Inclusion Bodies of Insect Viruses by Capillary Gas Chromatography
Abstract: A method for analysis of fatty acids in the insect inclusion body viruses by capillary gas chromatography was described. The main components of the fatty acid chromatogram were identified ,showing that the fatty acids in the granulosis virus and nuclear polyhedrosis virus were mainly composed of undecanoic a.,dodecanoic a.,tridecanoic a.,tetradecanoic a.,hexadecanoic a., heptadecanoic a., octadecanoic a., eicosanoic a. and octadecanoic a..Based on the ratios of the related fatty acid peak heights among distinct chromatograms,the viruses were differentiated and identified.The conclusion was consistent with that of the virus monosaccharide and pyrolysis gas chromatography.