庚型肝炎病毒NS5区蛋白鼠单克隆抗体的制备

Five hybridoma cell lines secreting monoclonal antibodies(McAb) against hepatitis G virus NS 5 protein (HGV NS5) were raised by fusing Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant HGV NS5 protein. The results of indirect ELISA demonstrated that these McAbs have no cross reaction with other antigenes, such as HBsAg, HCV C,HCV NS3 protein and E.coli lysate. The titers of hybridoma culture supernatant and ascitic fluid of mice were 1.28×10 2 -2.56×10 2 ,and 6.4×10 3 -1.28×10 5 ,respectively. These monoclonal antibodies could be used in histochemistry and diagnosis of HGV infection.