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Volume 13 Issue 3
June 1998
    Citation: WANG Ye-Fu, JI Xi-Peng, ZHU Ying, LI Xiao-Feng, LI Zhi-Da. An antiapoptosis p35 gene from Heliothis armigera polyhedrosis virus rescues Acp352 replication in non-permissible cell line .VIROLOGICA SINICA, 1998, 13(3) : 256.
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    An antiapoptosis p35 gene from Heliothis armigera polyhedrosis virus rescues Acp352 replication in non-permissible cell line

    • 1.

      Institute of Virology．Wuhan University，Wuhan 430072

    • Available online: 05 September 1998
    • p35 gene from baculovirus is an antiapoptosis gene which can sustain virus multiplication when infected by baculovirus. Both p35 deleted and inserting inactivated AcNPV can not replicate in Sf cell lines and Ha cell line.It is almost the same in BmNPV.Acp35Z which generated from AcNPV inserted LacZ gene whthin p35 gene causes apoptosis in Ha cell line ,but the abortion replication can be rescued by transient expression of HaNPV p35 gene drived by AcNPV IE1 promotor.The result was reconfirmed with X-gal manifest and Dot-ELISA.
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      An antiapoptosis p35 gene from Heliothis armigera polyhedrosis virus rescues Acp352 replication in non-permissible cell line

      • 1. Institute of Virology．Wuhan University，Wuhan 430072

      Abstract: p35 gene from baculovirus is an antiapoptosis gene which can sustain virus multiplication when infected by baculovirus. Both p35 deleted and inserting inactivated AcNPV can not replicate in Sf cell lines and Ha cell line.It is almost the same in BmNPV.Acp35Z which generated from AcNPV inserted LacZ gene whthin p35 gene causes apoptosis in Ha cell line ,but the abortion replication can be rescued by transient expression of HaNPV p35 gene drived by AcNPV IE1 promotor.The result was reconfirmed with X-gal manifest and Dot-ELISA.

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