Sequence determination of the specific DNA fragment from the genome of strong virulent GA strain of the Marek's disease virus
Abstract: Differentiation of strong virulent and vaccinal Marek's disease virus (MDV) was carried out by using DNA molecular hybridization. DNA probe was made of BamHI L fragment from MDV gene library (strain GA) by labelled with digoxigenin using random priming. And the complete nucleotide sequence of BamHI L fragment was further determined from subcloned DNAs by using double stranded sequencing method. The results of computer analysis showed that, (1) the entire DNA fragment is 2893 bp long and has a base composition of 55.91% G+C;(2) it has no any open reading frames (ORFs); (3) this DNA fragment is possibly involved in transcription of meq gene of MDV.