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Volume 13 Issue 4
August 1998
    Citation: WANG Bing, KE Li-Hua, JIANG Hong, TIAN Po, LI Chuan-Zhao. The Screen of Inhibitors of Grass Carp Hemorrhage Virus from a Phage Displayed Random Peptide Library .VIROLOGICA SINICA, 1998, 13(4) : 351.
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    The Screen of Inhibitors of Grass Carp Hemorrhage Virus from a Phage Displayed Random Peptide Library

    • 1.

      Wuhan Institute of Virology，Chinese Academy of Sciences．Wuhan 430071Institute of Microbiology．Chinese Academy of Sciences Beijing 100080

    • Available online: 05 December 1998
    • Screening inhibitors of GCHV 873 (Grass Carp Hemorrhage Virus)to inhibit its replication in CIK cells in vitro ,the biotinylated intact virion of GCHV was used to react on a random sequenced peptide library of 9 amino acids fused on protein Ⅲ of filamentous phage fUSE5 .After 3 rounds of affinity selection,immuno screening and ELISA,16 positive clones were selected using CsCl gradient purified virus particles, 6 of them inhibited the replication of GCHV in CIK cells in vitro and decreased the TCID 50 of GCHV from 10 -10 to 10 -5 .These findings open possibility of identifying virus proteins or epitopes as targets for antiviral agents.
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      The Screen of Inhibitors of Grass Carp Hemorrhage Virus from a Phage Displayed Random Peptide Library

      • 1. Wuhan Institute of Virology，Chinese Academy of Sciences．Wuhan 430071Institute of Microbiology．Chinese Academy of Sciences Beijing 100080

      Abstract: Screening inhibitors of GCHV 873 (Grass Carp Hemorrhage Virus)to inhibit its replication in CIK cells in vitro ,the biotinylated intact virion of GCHV was used to react on a random sequenced peptide library of 9 amino acids fused on protein Ⅲ of filamentous phage fUSE5 .After 3 rounds of affinity selection,immuno screening and ELISA,16 positive clones were selected using CsCl gradient purified virus particles, 6 of them inhibited the replication of GCHV in CIK cells in vitro and decreased the TCID 50 of GCHV from 10 -10 to 10 -5 .These findings open possibility of identifying virus proteins or epitopes as targets for antiviral agents.

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