Constructural analysis of antiviral peptide to grass carp hemorrhage virus

Through the reaction between GCHV 873 (Grass Carp Hemorrhage Virus) particles and random peptide library displayed on phage in vitro, after 3 rounds of affinity selection 16 phage clones from 300 clones have been got, which have high affinity with GCHV. Six of sixteen clones could inhibit the replication of GCHV in CIK in vitro and decrease the TCID 50 to half of index. The DNA sequence of phage clones and deduced sequence of amino acid of the inserted foreign fragments were analysed. The results show that the sequences of six clones are identical. The deduced sequence of amino acid is NH2 Leu Trp Val Gly Gly Gly Arg Asn Ala. It indicates that the ability of anti GCHV is certainly related to specific structural information and amino acid sequence of peptides. The determination of antiviral peptide offers a fundamental practical advance in the synthesis of the antiviral peptides and provides a way of generating noval antiviral agents.