M olecular Cloning and Sequencing the NS5 Genomic Region of HGV Guangdong and Hong Kong Strains

HGV NS5 cDNA of two Hong Kong strains and one Guangdong strain were amplified by re verse transcription polymerase chain reaction (RT-PCR)．T}le products were inserted into pUC19 vect0rs respectively．~fter transfecting DH5a and JM 109 strains．the reeombinants were screened and identified by PCR and dig ted wlth restriction erldDnudeses．n 1e nudeotldes were sequenced by the Ndmxy chain termination method n 1e homologies d HGV NS5 r~ ion nucleotide and d~umdlTlillO acid sequences were 93 3％ 一94％ and 97％ 一99．2％ re印ecnve【v between eachHangKong strainandtheGtm~dong strain an dwere 90％ 一91 2％ and 94％ 一96 3％ ，87．1％ 一89．5％ an d 95．2％ 一97％ 91 4％ 93 8％ and 97％ 97 9％ respectively Compared with the reported China isolate(CN) American iso lates(PNF2161 and R10291)，and West African lsolated(GBV-C) T}1e variant sites of the nudeotides appear spor~ ieally T}le nucleotide an d ded umd amino acid seQuences of HGV NS5 genornic region are relatively conserved ，andit also sog gests thatthe nudeotide variation ofHGV may beinfluenced by geo graphical factors