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Volume 14 Issue 2
April 1999
    Citation: YANG Tie-Qiu, LIU Jian-Jun, XIAO Gong, WEN Li, GUO Chu-Fang, SONG Jie-Ping. Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells .VIROLOGICA SINICA, 1999, 14(2) : 106-109.
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    Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells

    • 1.

      Virus Research Institute，Huhei Medica[University， Wuhan 430071

    • Available online: 05 June 1999
    • The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay，reverse transcription polymerase chain reaction and cy tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec tion，vira1 antigen was positive at 8 hours and cytopathic effect could be found at 12 hours The vira1 particles could be observed at 20 hours in postinfeedon These results provided new data for observation of CBV propagation，also，it indicated that the reverse transcription polymerase chain reaction was a sensitive technique for m onitoring CBV propagation
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      Propagation Characteristics of Coxsackie B Virus in Hep一2 Cells

      • 1. Virus Research Institute，Huhei Medica[University， Wuhan 430071

      Abstract: The dynamic change of coxsackie B virus(CBV)5 type propagation was studied in Hep一2 cells by immunofluorescence assay，reverse transcription polymerase chain reaction and cy tological methods The results showed that CBV RNA muld be detected at 4 hours in postinfec tion，vira1 antigen was positive at 8 hours and cytopathic effect could be found at 12 hours The vira1 particles could be observed at 20 hours in postinfeedon These results provided new data for observation of CBV propagation，also，it indicated that the reverse transcription polymerase chain reaction was a sensitive technique for m onitoring CBV propagation

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