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Volume 14 Issue 2
April 1999
    Citation: LI Gong-Wei, LIU Xiang-Chao, LI Xiao-Bing, HAN Xue-Qing, YAN Shen. Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C .VIROLOGICA SINICA, 1999, 14(2) : 169-173.
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    Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C

    • 1.

      ChangchunUniversity ofAgricultural andAnimal Sciences,Changchun 130062

    • 2.

      Lanzhou Veterinary Institute，Lanzhou 730046

    • Available online: 05 June 1999
    • The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies on nucleotide and amino acid sequences were 99.08% and 98.42%, respectively.
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      Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C

      • 1. ChangchunUniversity ofAgricultural andAnimal Sciences,Changchun 130062
      • 2. Lanzhou Veterinary Institute，Lanzhou 730046

      Abstract: The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies on nucleotide and amino acid sequences were 99.08% and 98.42%, respectively.

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