The Assem bly and Release of Sim ian AIDS Type D Retrovirus （SRV)：Involvement of Intermediate Filament

By conventional EM technique and DGD embedment free section EM technique together with the procedure of gentle extraction, the assembly and release of simian AIDS type D retrovirus (SRV) in infected Raji cells were studied. The results showed that SRV nucleocapsids were assemblied in some cytoplasm areas near nuclei, and these areas or virus assembly centers were suspended in the intermediate filament network; and the assembling and assembled virus nucleocapsids were associated firmly with intermediate filaments. It is suggested that the assembly of SRV nucleocapsids depends on intermediate filaments as support, and the assembled nucleocapsids may move along the intermediated filaments to cytoplasm membrane where they bud and are released.