Citation: DIAO Jun, ZHANG Xiu-Gen, CHEN Bu-Yan, CA Bao-Xiang. Construction of a Recom binant Herpesvirus of Turkey Expressing the Haem agglutinin-Neuram inidase Gene of Newcastle Disease Virus .VIROLOGICA SINICA, 1999, 14(3) : 259-264.

Construction of a Recom binant Herpesvirus of Turkey Expressing the Haem agglutinin-Neuram inidase Gene of Newcastle Disease Virus

  • Available online: 05 September 1999
  • A 1.8 kb fragment of Newcastle disease virus (NDV) haemagglutinin neuraminidase (HN) gene was amplified by polymerase chain reaction (PCR) with primers containing enzyme cleavage sites, and inserted into a nonessential gene of herpesvirus of turkey (HVT). Recombinant viruses were screened by Dot blot hybridization with digoxigenin labelled probe and purified by plaque formation technique. The expression of NDV HN gene can be detected by Western blotting in CEF cells infected with recombinant virus. Recombinant HVT strains were replicated stably in cell cultures.

  • 加载中
  • 加载中

Article Metrics

Article views(3885) PDF downloads(952) Cited by()

Related
Proportional views

    Construction of a Recom binant Herpesvirus of Turkey Expressing the Haem agglutinin-Neuram inidase Gene of Newcastle Disease Virus

    • 1. ColLege ofAnimal Medicine,Nanjing Agricultural University,Nanljlng 210095

    Abstract: A 1.8 kb fragment of Newcastle disease virus (NDV) haemagglutinin neuraminidase (HN) gene was amplified by polymerase chain reaction (PCR) with primers containing enzyme cleavage sites, and inserted into a nonessential gene of herpesvirus of turkey (HVT). Recombinant viruses were screened by Dot blot hybridization with digoxigenin labelled probe and purified by plaque formation technique. The expression of NDV HN gene can be detected by Western blotting in CEF cells infected with recombinant virus. Recombinant HVT strains were replicated stably in cell cultures.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return