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Volume 14 Issue 4
August 1999
    Citation: LUO Bao-Jun, WANG Hua-Lin, CHEN Xin-Wen, SUN Xiu-Lan, WANG Han-Zhong, BANG Hui-Yin, HU Zhi-Hong, Basil M.Arif, Just M.Valk. Genetic Organization of the BamHI-H Region of the Single Nucleocapsid Nucleopolyhedrovirus of Buzura suppressaria (BusuNPV) .VIROLOGICA SINICA, 1999, 14(4) : 333-342.
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    Genetic Organization of the BamHI-H Region of the Single Nucleocapsid Nucleopolyhedrovirus of Buzura suppressaria (BusuNPV)

    • 1.

      Joint-lab of invertebrate Virology，Wuhan Institute of Virology，Chinese Academy of Sciences，Wuhan 430071

    • Available online: 05 December 1999
    • In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the BamHI-H fragment located at mapunits (mu)81. 6-83. 6 of the viral genome was sequenced. The fragment contained two partialand one complete open reading frames (ORFs) representing the 5' end of a p47 gene, a cathepsingene and the 3' end of a p74 gene. Sequence analysis futher revealed that these ORFs have thesame conserved motifs and gene structure as those observed in their homologues from other baculoviruses. The genomic arrangement of the ORFs in the BusuNPV BamHI-H fragment is verydifferent from the arrangement of their homologues in the genome of Autographa californica mutiple nucleocapsid (M) NPV.
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      Genetic Organization of the BamHI-H Region of the Single Nucleocapsid Nucleopolyhedrovirus of Buzura suppressaria (BusuNPV)

      • 1. Joint-lab of invertebrate Virology，Wuhan Institute of Virology，Chinese Academy of Sciences，Wuhan 430071

      Abstract: In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the BamHI-H fragment located at mapunits (mu)81. 6-83. 6 of the viral genome was sequenced. The fragment contained two partialand one complete open reading frames (ORFs) representing the 5' end of a p47 gene, a cathepsingene and the 3' end of a p74 gene. Sequence analysis futher revealed that these ORFs have thesame conserved motifs and gene structure as those observed in their homologues from other baculoviruses. The genomic arrangement of the ORFs in the BusuNPV BamHI-H fragment is verydifferent from the arrangement of their homologues in the genome of Autographa californica mutiple nucleocapsid (M) NPV.

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