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Volume 15 Issue 2
April 2000
    Citation: WEI Ta-Yun, LIN Han-Xin, TUN Jie-Jian, LIN Ai-Yang, XIE Lian-Hui. Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4 .VIROLOGICA SINICA, 2000, 15(2) : 156-162.
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    Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4

    • 1.

      Institute of Plant Virologyo of Fujian Agricuhurat University ,Key Laboratory of Plant Virology of Fujian Province，Fuzhou 350002,China

    • Available online: 05 June 2000
    • The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. Computer assisted folding analysis showed that the intergenic region was rich in A and U residues where two distant hairpin structures could be formed. One was highly conserved and stable, but the other was rather unstable because of bases variation. Therefore, there were molecular variation among isolates of rice stripe virus in China.
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      Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4

      • 1. Institute of Plant Virologyo of Fujian Agricuhurat University ,Key Laboratory of Plant Virology of Fujian Province，Fuzhou 350002,China

      Abstract: The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. Computer assisted folding analysis showed that the intergenic region was rich in A and U residues where two distant hairpin structures could be formed. One was highly conserved and stable, but the other was rather unstable because of bases variation. Therefore, there were molecular variation among isolates of rice stripe virus in China.

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