Som e M ultiplication Properties of the Lapinized Chinese Strain(C strain)of Classical Swine Fever Virus in Prim ary Bovine Testicular Cells

Some multiplication properties of the Lapinized Chinese strain of Classical Swine Fever Virus (CSFV C strain) in primary bovine testicular cells were studied. One CSFV C strain which can be detected by immunofluorescence technique was found and a new method in titering called flurescent plaque formation unit (F PFU) was developed. It took about 5 days for all cells of primary bovine testis to be infected by CSFV and the titre of progeny virions in medium was 10 5 F PFU/ml from then on. The cell types of cultured bovine testicular cells changed along with cell passages. The mechanism of some phenomena in vaccine production was showed and some factors which effect the multiplication of CSFV C strain in primary bovine testicular cells were studied. Aknowledgements on the multiplication of CSFV C strain in primary bovine testicular cells were enriched and a new way to raise the titer of CSFV was suggested.