Citation: DIAO Zhen-Fen, HUANG E-Dong, WANG Lin-Chuan, LIU Gong-Beng, XIN Chao-An. Study on RT-PCR/RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain .VIROLOGICA SINICA, 2000, 15(2) : 197-199.

Study on RT-PCR/RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain

  • Available online: 05 June 2000
  • A 1.66 kb expected S1 gene fragment of avian infectious brochitis virus (IBV) H strain was amplified by RT PCR. Its PCR/RFLP pattern was similar to IBV D 41 strain and M 41 strain when digested using BstYI, HaeⅢ and EcoRI respectively. IBV H strain was thought as Massachusetts serotype.

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    Study on RT-PCR/RFLP of Spike Protein S1 Gene of Avian Infectious Brochitis Virus H Strain

    • 1. College of Zhenzhou Animal Husbandry,Zhenzhou 450008,China

    Abstract: A 1.66 kb expected S1 gene fragment of avian infectious brochitis virus (IBV) H strain was amplified by RT PCR. Its PCR/RFLP pattern was similar to IBV D 41 strain and M 41 strain when digested using BstYI, HaeⅢ and EcoRI respectively. IBV H strain was thought as Massachusetts serotype.

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