Citation: LIAO Guo-Yang, JIANG Shu-De, ZHENG Cong-Xi, LI Wei-Dong, WANG Meng-Xiu, CHEN Guang-Wu, WANG Yan. Studies on the Purification of OPV and its Characterization .VIROLOGICA SINICA, 2000, 15(3) : 226-231.

Studies on the Purification of OPV and its Characterization

  • Available online: 05 September 2000
  • Q Sepharose F.F. was adopted to purify the suspension of oral poliovaccine (OPV), which is prepared from Vero cells. After clarification and concentration by ultrafiltration, the recovery of virus infected titre may attain above 85%. Using column chromatography on Q Sepharose F.F. to purify the concentrated virus suspension, the purified viruses attain 100% recovery of virus infectivity. Dot membrane hybridization was used to detect DNA with the probe of Vero cell genome DNA which was labed with 32 P dATP, the contents of residual substrate DNA was less than 100 pg/dose. The process of downstream had no significant influence on some biologiccal characters of purified viruses, such as virus morphology, tumorigenicity, rct/40 character and capsid protein. The results indicate that Q Sepharose F.F. is an ideal chromatography material for purifying OPV prepared from Vero cells.

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    Studies on the Purification of OPV and its Characterization

    • 1. Institute of Medical Biology,Chinese Academy of Medical Science&Peking Union of Medical College,Kunming 650107,China

    Abstract: Q Sepharose F.F. was adopted to purify the suspension of oral poliovaccine (OPV), which is prepared from Vero cells. After clarification and concentration by ultrafiltration, the recovery of virus infected titre may attain above 85%. Using column chromatography on Q Sepharose F.F. to purify the concentrated virus suspension, the purified viruses attain 100% recovery of virus infectivity. Dot membrane hybridization was used to detect DNA with the probe of Vero cell genome DNA which was labed with 32 P dATP, the contents of residual substrate DNA was less than 100 pg/dose. The process of downstream had no significant influence on some biologiccal characters of purified viruses, such as virus morphology, tumorigenicity, rct/40 character and capsid protein. The results indicate that Q Sepharose F.F. is an ideal chromatography material for purifying OPV prepared from Vero cells.

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