Screening of DNA Polymerase Gene from W hite Spot Syndrome Virus (WSSV)by Using Degenerated Oligonueleotide

Degenerated oligonucleotides were designed from one motif VYGDTDS of several viral DNA polymerases and were labelled with Digoxigenin to screen DNA polymerase gene in a White Spot Syndrome Virus (WSSV) DNA library. One Eco R I fragment of 707 bp was selected and contains a putative open reading frame in which the motif YGDTDS of family B of DNA polymerase was found. This selected sequence was subsequently compared with GenBank data. The results showed that this deduced amino acids sequence has some similarities with several algal like virus of the family Phycodnavirus. So we deduced that this is one DNA polymerase gene fragment of WSSV.