Serving the Society Since 1986
Advanced Search
Volume 15 Issue 4
August 2000
    Citation: DIAO Dong-Lan, SHU Shui-Fang, HUANG Wen-Qing, ZHANG Man-Liang. Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene .VIROLOGICA SINICA, 2000, 15(4) : 357-360.
    shu

    Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene

    • 1.

      Xuzhou AgriculturatResearch Institute，Xuzhou．Jiangsu，221121 China

    • Available online: 05 December 2000
    • A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of GLRaV 3 from America. The expected size of CP gene of GLRaV 3 was amplified by IC (Immuno capture) RT PCR method. The CP gene of GLRaV 3 was cloned into pGEM 3zf(+) vector and sequenced. The nucleotide sequence was compared with American isolate. Result showed that their homology was 94.1%.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(3695) PDF downloads(772) Cited by()

    Related
    Proportional views

      Cloning of Grapevine Leafroll Associated Virus 3 Coat Protein Gene

      • 1. Xuzhou AgriculturatResearch Institute，Xuzhou．Jiangsu，221121 China

      Abstract: A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of GLRaV 3 from America. The expected size of CP gene of GLRaV 3 was amplified by IC (Immuno capture) RT PCR method. The CP gene of GLRaV 3 was cloned into pGEM 3zf(+) vector and sequenced. The nucleotide sequence was compared with American isolate. Result showed that their homology was 94.1%.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return