Com parative Analysis of Serological and M olecular M ethods for the detection of Rice Grassy Stunt Virus

Methods of ELISA, nonradioactive molecular hybridization and RT PCR were applied in the detection of rice grassy stunt virus (RGSV). The detection sensitivity of indirect ELISA using antiserum against fusion protein GST NC was 1 mg of infected leaves or 84 ng of purified virus. The method of dot hybridization using NC, a DIG labelled DNA probe was 50 g diseased leaves, or 6 ng purified preparations. The detection endpoint of RT PCR was 10 g diseased leaves, or 2 ng purified virus preparation. Comparisons of sensitivity and maneuverability were made among these methods.