Molecular Cloning and Sequencing of VP2 Gene of Canine Parvovirus Inner Mongolia Isolate(CPV-IM)

The DNA of Canine Parvovirus Inner Mongolia isolate (CPV IM) was isolated from the enteric lysate of virus infected dog showing enteritis symptoms. The VP2 gene of CPV IM was amplified by PCR using designed and synthesized two primers and cloned into plasmid pUC19 at Bam H I/ Sac I sites. Recombinant plasmid pUCVP2 was identified by PCR and restriction enzymes analysis and nucleotide sequence analysis. Results show that the full length VP2 gene clone of CPV IM was obtained. VP2 gene of CPV IM isolate consists of 1 755 nt, and there is a high homology in nucleotide sequence and amino acid sequence in comparison with CPV N, CPV B and Feline Panleukopenia Virus (FPLV). The rate of nucleotide sequence homology is 99.32%, 98.29% , 98.52%, and the rate of amino acid homology is 98.97%, 97.09%, 97.77%, respectively.