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Volume 15 Issue 4
August 2000
    Citation: LI Guang-Fu, TANG Jia-Qi, CHEN Yu-Ping, WANG Yan, CAO Min, LI Xian-Fu, BO Xiu-Zhen. Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv .VIROLOGICA SINICA, 2000, 15(4) : 409-413.
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    Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv

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    • Available online: 05 December 2000
    • The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.
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      Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv

      • 1. Institute of 

      Abstract: The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.

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