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Volume 16 Issue 1
February 2001
    Citation: W ANG Youchun。, ZHANG Hua—yuan, GU Wen—jie ．Roger Ling ．LI He-min。．Tim J Harrison, . Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV) .VIROLOGICA SINICA, 2001, 16(1) : 28-33.
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    Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)

    • 1.

      1 Natiottal Institute for the Control of Pharmaceutical and Biological Products，Beijing 100050．China

    • 2.

      Center for Hepatology，Royal Free Campus，University College London，Hamstead．London NW3 2PF，UK

    • Available online: 05 February 2001
    • ORF3 and partial ORF1 regions were amplified with RT-PCR from ta~vo patients(T1 and TII)infected with new genotype of hepatitis E Virus．The PCR products were cloned and sequenced The results showed that G-C rich region in ORF3 was deleted when amplified with norrflal PCR reac— tion However．PCR reaction containing G-C melt solution can overcome this problem The sequence analysis showed that T1 and Tll belong tO a new genotype of HEV which differs from genotype I，II and III reported．T1 and T11 have 79％ -82％ ．80％ -81％ and 83％ ～85％ identical tO genotype I． II and Ill respectively
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      Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)

      • 1. 1 Natiottal Institute for the Control of Pharmaceutical and Biological Products，Beijing 100050．China
      • 2. Center for Hepatology，Royal Free Campus，University College London，Hamstead．London NW3 2PF，UK

      Abstract: ORF3 and partial ORF1 regions were amplified with RT-PCR from ta~vo patients(T1 and TII)infected with new genotype of hepatitis E Virus．The PCR products were cloned and sequenced The results showed that G-C rich region in ORF3 was deleted when amplified with norrflal PCR reac— tion However．PCR reaction containing G-C melt solution can overcome this problem The sequence analysis showed that T1 and Tll belong tO a new genotype of HEV which differs from genotype I，II and III reported．T1 and T11 have 79％ -82％ ．80％ -81％ and 83％ ～85％ identical tO genotype I． II and Ill respectively

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