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Volume 16 Issue 1
February 2001
    Citation: ZHANG Dongwei, LIAN G, QI Zi, LING Shi. HCVE2区基因的分子克隆及序列分析 .VIROLOGICA SINICA, 2001, 16(1) : 40-44.
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    HCVE2区基因的分子克隆及序列分析

    • 1.

      1 Wuhan Institute of Virology，Academia Sinica，Wuhan 430071．China’ 2 1p bf Hepatitis．,National Institutefor the Control ofPharmaceutical and Biology Produc~，8dj~g 100050．China

    • Available online: 05 February 2001
    • HCV RNA positive serum was first selected by RT PCR test kit from several anti HCV positive sera obtained from Xi’an.HCV RNA extracted from the elected sera was converted to cDNA by reverse transcription with random primer.Half nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET 22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates reported previously showed that the sequence belonged to HCV type Ⅱ.
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      HCVE2区基因的分子克隆及序列分析

      • 1. 1 Wuhan Institute of Virology，Academia Sinica，Wuhan 430071．China’ 2 1p bf Hepatitis．,National Institutefor the Control ofPharmaceutical and Biology Produc~，8dj~g 100050．China

      Abstract: HCV RNA positive serum was first selected by RT PCR test kit from several anti HCV positive sera obtained from Xi’an.HCV RNA extracted from the elected sera was converted to cDNA by reverse transcription with random primer.Half nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET 22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates reported previously showed that the sequence belonged to HCV type Ⅱ.

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