Citation: QIN Ai, CUI Zhi, Lucy Lee, Aly Fadly. Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus .VIROLOGICA SINICA, 2001, 16(1) : 68-73.

Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus

  • Available online: 05 February 2001
  • The envelope gene of ADOL-4817 strain of avian leukosis v[ros subgroup J(ALV—J)was amplified by polymerase chain reaction(PCR)and cloned into TA vector.The sequence analysis re— suits showed that tbe envelope gene is composed of 1 746 bp.1 554 bp of which could be translated in— to 517 amino acids for gp85 and gp37.The molecular weight of envelope protein is 57.7kD There are I5 potential glycosy[ation sites in the envelope protein,13 of which is located in gp85,Analysis of se— quences of envelope gene indicate that ADOL-48 17 showed high degree of sequence identity to other ALV—J strains,and most closely related to the like—envelope gene of endogenous virus EAV—HP but di— vergent from these of other ALV subgroup A-E .These data support the hypothesis that envelope gene of avian leukosis virus suhgroup J maybe acquired hy l:ecombination with expressed sequences

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    Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus

    • 1. 1.Dept of Veterinary Medicine, Yangzhou Uniwersity,Yangzhou 225009·China 2 Avian disease and ontology laboratory.USDA.East lansing,MI 48823 USA

    Abstract: The envelope gene of ADOL-4817 strain of avian leukosis v[ros subgroup J(ALV—J)was amplified by polymerase chain reaction(PCR)and cloned into TA vector.The sequence analysis re— suits showed that tbe envelope gene is composed of 1 746 bp.1 554 bp of which could be translated in— to 517 amino acids for gp85 and gp37.The molecular weight of envelope protein is 57.7kD There are I5 potential glycosy[ation sites in the envelope protein,13 of which is located in gp85,Analysis of se— quences of envelope gene indicate that ADOL-48 17 showed high degree of sequence identity to other ALV—J strains,and most closely related to the like—envelope gene of endogenous virus EAV—HP but di— vergent from these of other ALV subgroup A-E .These data support the hypothesis that envelope gene of avian leukosis virus suhgroup J maybe acquired hy l:ecombination with expressed sequences

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