Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus
Abstract: The envelope gene of ADOL-4817 strain of avian leukosis v[ros subgroup J(ALV—J)was amplified by polymerase chain reaction(PCR)and cloned into TA vector.The sequence analysis re— suits showed that tbe envelope gene is composed of 1 746 bp.1 554 bp of which could be translated in— to 517 amino acids for gp85 and gp37.The molecular weight of envelope protein is 57.7kD There are I5 potential glycosy[ation sites in the envelope protein,13 of which is located in gp85,Analysis of se— quences of envelope gene indicate that ADOL-48 17 showed high degree of sequence identity to other ALV—J strains,and most closely related to the like—envelope gene of endogenous virus EAV—HP but di— vergent from these of other ALV subgroup A-E .These data support the hypothesis that envelope gene of avian leukosis virus suhgroup J maybe acquired hy l:ecombination with expressed sequences