Serving the Society Since 1986
Advanced Search
Volume 16 Issue 1
February 2001
    Citation: ZHANG Hai, ZHANG Yun-zhi, HUANG W, MI Zhu, GONG He-qin, WANG Jing. Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province .VIROLOGICA SINICA, 2001, 16(1) : 74-77.
    shu

    Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province

    • 1.

      Yunnan Province Institute of Epidemic Diseases Control and Research，Oali 671000，China

    • Available online: 05 February 2001
    • In July 1997, a strain (GB30) of virus was isolated from 60 samples of brain tissues of Murina aurata (Chiroptera: Vespertilionidae) collected in Gengma county, Yunnan province, China. Isolation of virus was negative from 4 samples of brain tissues of Rousettus leschenaulti (Chiroptera: Pteropodidae) collected in Gengma. GB30 virus strain could regularly cause illness and death in suckling mice, produced evident CPE in BHK21 cells. It agglutinated red blood cells of dove at pH5.75～7.4. This virus has been identified serologically by hemagglutination inhibition and immunofluorescent tests using Japanese encephalitis (JE), dengue (DEN) type 1,2,3,4, and chikungunya (CHIK) viruses monoclonal antibodies, and JE and sindbis (SIN) viruses immune sera. It showed specific reaction to JE virus only and no reaction with DEN 1～4, CHIK and SIN viruses. Therefore it can be identified as JE virus. This is the first report on the isolation of JE virus from Murina aurata . The results showed that bats are consider
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(3973) PDF downloads(988) Cited by()

    Related
    Proportional views

      Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province

      • 1. Yunnan Province Institute of Epidemic Diseases Control and Research，Oali 671000，China

      Abstract: In July 1997, a strain (GB30) of virus was isolated from 60 samples of brain tissues of Murina aurata (Chiroptera: Vespertilionidae) collected in Gengma county, Yunnan province, China. Isolation of virus was negative from 4 samples of brain tissues of Rousettus leschenaulti (Chiroptera: Pteropodidae) collected in Gengma. GB30 virus strain could regularly cause illness and death in suckling mice, produced evident CPE in BHK21 cells. It agglutinated red blood cells of dove at pH5.75～7.4. This virus has been identified serologically by hemagglutination inhibition and immunofluorescent tests using Japanese encephalitis (JE), dengue (DEN) type 1,2,3,4, and chikungunya (CHIK) viruses monoclonal antibodies, and JE and sindbis (SIN) viruses immune sera. It showed specific reaction to JE virus only and no reaction with DEN 1～4, CHIK and SIN viruses. Therefore it can be identified as JE virus. This is the first report on the isolation of JE virus from Murina aurata . The results showed that bats are consider

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return