Expression of P 19 Gene of Avian Leukosis Virus in Escherichia coli

Abstract：Based on avian 1eukosis virus(ALV)p19 gene termina1 nucleotide sequence，a 82 bp dou— ble．stranded DNA fragment was chemically synthesized and cloned into the expression eect~r pGE— M EX．1 The sequencing result indicated that the cloned fragmentⅥw a correct version of the one de— signed both in nucleotide sequence and in its open reading frame．The recombinant was used to transfo／ Tn E．∞“ BL21(DE3) The cloned fragment was expressed as a fused protein with T7 gene 10 leader peptide and was shown to be 34 kD in size on SDS—PAGE ge1 when induced with l mmol／L IPTG．The expression product was able to bind immunologically to rabbit anti—ALV serum in W est— ern-blot assay and is being tested to differentiate exogenous from endogenous ALV ．