Citation: HUANG Can-hua, SHI Zheng, ZHANG Lu, XIE Yun-li, ZHANG Li, CHEN Di, wU Qing-jiang. Homology Comparison of White Spot Syndrome Baculovirus(WSSV) from Penaeid shrimp .VIROLOGICA SINICA, 2001, 16(1) : 81-84.

Homology Comparison of White Spot Syndrome Baculovirus(WSSV) from Penaeid shrimp

  • Available online: 05 February 2001
  • Homology of three WSSV isolates.which were sampled from representative maritime space of China:Tanghai isolate(13o Bay of China),Ningbo isolate(East China Sea).Shenzhen isolate (South China Sea)was compared.Both of the genome RFLP patterns and the characteristic structural proteins SDS-PAGE electropherograms showed that they were quite$a/’ae.It suggested that they were the same kind of WSSV virus that caused explosive epidemic diseases of shrimps(EEDs)throughout southern and northern China The$drne large PCR products achieved when using the PCR primers from RV—PJ:PRDV (P japonicus.Japan)and WSBV=PmNOBIII(P.morR~don Taiwan,Chi— na)respectively to amplify the genome from P.chinensis(Tanghai,China)with high fidelity Taq Polymerase.The sequence identities of WSSV from P.chinens~ with those from RV—PJ=PRDV (P 血ponicus,Japan)and WSBV=PmNOBill(P.monodon Taiwan.China)are 97% and 100% respectively,the results provided additiona l evidence that W SSV reported in different parts of the Asian and Pacific regions maybe quite the same or jLISt different variants of the same virus

  • 加载中
  • 加载中

Article Metrics

Article views(4102) PDF downloads(770) Cited by()

Related
Proportional views

    Homology Comparison of White Spot Syndrome Baculovirus(WSSV) from Penaeid shrimp

    • 1. 1 State Key Laboratory ofFresh,raterEcology andB~otechnology.1nstituteof Hydrohiology,Academia Sinica, Wuhan 430072.China
    • 2. Jointtaboratot~+of InvetebrateVirology.WuhanInstitute VirologyAcademia Sinica,Wuhan 43007I.China

    Abstract: Homology of three WSSV isolates.which were sampled from representative maritime space of China:Tanghai isolate(13o Bay of China),Ningbo isolate(East China Sea).Shenzhen isolate (South China Sea)was compared.Both of the genome RFLP patterns and the characteristic structural proteins SDS-PAGE electropherograms showed that they were quite$a/’ae.It suggested that they were the same kind of WSSV virus that caused explosive epidemic diseases of shrimps(EEDs)throughout southern and northern China The$drne large PCR products achieved when using the PCR primers from RV—PJ:PRDV (P japonicus.Japan)and WSBV=PmNOBIII(P.morR~don Taiwan,Chi— na)respectively to amplify the genome from P.chinensis(Tanghai,China)with high fidelity Taq Polymerase.The sequence identities of WSSV from P.chinens~ with those from RV—PJ=PRDV (P 血ponicus,Japan)and WSBV=PmNOBill(P.monodon Taiwan.China)are 97% and 100% respectively,the results provided additiona l evidence that W SSV reported in different parts of the Asian and Pacific regions maybe quite the same or jLISt different variants of the same virus

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return