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Volume 16 Issue 2
April 2001
    Citation: CHEN Chen, LIU Kun, WANG Jin zhong, IVIA Ming, CENG Yun-qi CHEN Qi min ．ZHU Xiao-ke ．XIA Jian-hui, . High Expression，Purification and Imm uno-indentification of Recombinant HIV Surface Antigen gpl20 .VIROLOGICA SINICA, 2001, 16(2) : 109-113.
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    High Expression，Purification and Imm uno-indentification of Recombinant HIV Surface Antigen gpl20

    • 1.

      1 College of Life Sciences，Nankai University-Tiawin 300071 China

    • Available online: 05 June 2001
    • Different—length fragments of the HIV trans-membrane antigen (gp120)gene were ex pressed in Escherichia coti，using T7 expression system ．SDS-PAGE stained by Coomassic Brilliant Blue showed no expression of full—length gpl20 and poor expresion 0f half—length gpl20 fragments from the N—terminal，but high expression of 1／3一lgenth gpl20 gene fragments from the N term inal (including V1／V2 epitopes)．more than 18％ of total bacterial protein Western blot showed fairly good reactivity to serum from HIV-infected individua1．On this basis，we expresed the corresponding tp 120 fragments of the HIV strains epidemic in China This study laid a solid foundation for expIo— ration of high expression of gP 120 in E coli and development of HIV serological diagnostic system for Chinese people．
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      High Expression，Purification and Imm uno-indentification of Recombinant HIV Surface Antigen gpl20

      • 1. 1 College of Life Sciences，Nankai University-Tiawin 300071 China

      Abstract: Different—length fragments of the HIV trans-membrane antigen (gp120)gene were ex pressed in Escherichia coti，using T7 expression system ．SDS-PAGE stained by Coomassic Brilliant Blue showed no expression of full—length gpl20 and poor expresion 0f half—length gpl20 fragments from the N—terminal，but high expression of 1／3一lgenth gpl20 gene fragments from the N term inal (including V1／V2 epitopes)．more than 18％ of total bacterial protein Western blot showed fairly good reactivity to serum from HIV-infected individua1．On this basis，we expresed the corresponding tp 120 fragments of the HIV strains epidemic in China This study laid a solid foundation for expIo— ration of high expression of gP 120 in E coli and development of HIV serological diagnostic system for Chinese people．

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