Citation: GONG Rui, MA Hul—wen, TONG Li—heng. Expression in E .Coti and Antigenicity of HGV NS5 Protein .VIROLOGICA SINICA, 2001, 16(2) : 114-118.

Expression in E .Coti and Antigenicity of HGV NS5 Protein

  • Available online: 05 June 2001
  • 880 bp cD A localized to the putative NS5 region of HGV genome was expressed in E. colf BL2I(DE3) The eDNA fragment was inserted into a plasmid pGEX-5X一1,at the downstream of the DNA sequence encoding Schistosoma japonicum glutathioue S_transferase(GST),in the S~TIe reading frame with the gene of GST.A 60KD GS丁_ S53 fusion protein was expressed at 37℃ in a forill of nclusion bodies amounting to 30 percent of total host protein whereas at 20℃ mainly in a form of soluble pmtein The fusion protein was extracted and purified to homologue,The purified GST— NS53 fusion protein could be specifically recognized with either the sera from the patient infected by HGV or the antisera directed against GST

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    Expression in E .Coti and Antigenicity of HGV NS5 Protein

    • 1. 1.College of Life Sciences,Wuhan University,Wuhan 430072,China2 Wuhan R&D Center for Biotechnology,Wuhan 430033,China

    Abstract: 880 bp cD A localized to the putative NS5 region of HGV genome was expressed in E. colf BL2I(DE3) The eDNA fragment was inserted into a plasmid pGEX-5X一1,at the downstream of the DNA sequence encoding Schistosoma japonicum glutathioue S_transferase(GST),in the S~TIe reading frame with the gene of GST.A 60KD GS丁_ S53 fusion protein was expressed at 37℃ in a forill of nclusion bodies amounting to 30 percent of total host protein whereas at 20℃ mainly in a form of soluble pmtein The fusion protein was extracted and purified to homologue,The purified GST— NS53 fusion protein could be specifically recognized with either the sera from the patient infected by HGV or the antisera directed against GST

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