Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China
Abstract: To determine the complete nuelcotide sequence of the S genome segment of HTN261 virus strain which was isolated from the striped field mouse(Apodemus agraius)in Heilongjiang province of the Northeast China．the complete nucleotide sequence of the S genome segment of HTN261 virus strain was determined with two clones，which were obtained using RT-PCR，and by linking two over lapping fragments into PUC19 vector respectively．The complete nueleotide sequence of the S genome segment of HTN261 virus strain WaS 1697 nucleotides in length and contained one ORF encoding the N protein starts at position 37 and ends at position 1326 The length of predicted protein，429 amino acids，is identieal tO the N protein of HTN，DOB，SEO types of hantaviruses Comparison of S seg— ment sequence of HTN261 strain with the corresponding sequences of other hantaviruses showed that the dentities of HTN261 strain with HTN76—118 strain were 89％ (complete nueleotide sequence) andd 98％ (amino acid sequence)．Phylogenetie ana lysis showed that HTN261 strain with HTN76— 1 18 strain form one lineage within the clade containing hantaviruses of HTN type．But the identities of HTN261 strain with HTN76—1 18 strain differ by 1 1％ in complete nucleotide sequence of S segment and 2％ in amino acid sequence of N protein．HTN261 strain belongs to hantavirus HTN type and may be a new subtype
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